AIF1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb704471
• Category: Antibodies
• Description: AIF1 Recombinant Rabbit Monoclonal Antibody
• Target: AIF1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human IBA1 (1-38aa)
• UniProt ID: P55008
• MW: 17 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: 9A3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AIF 1; AIF1; AIF1 protein; IBa1; iba-1; IBa 1; all
• Research Area: Actin Binding Proteins, Cell Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug total protein per lane of various lysates probed with AIF1 (9A3) monoclonal antibody, unconjugated (orb704471) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Small Intestine, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with AIF1 (9A3) Monoclonal Antibody, Unconjugated (orb704471) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.