AIF1 Antibody

SKU: orb1247863

Description

AIF1 Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 17kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 0.5-1.5µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Mouse Brain shows staining of astrocytes. Recommended concentration: 2-4µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10µg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NKQFLDDPKYSSDED
• Target: AIF1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

AIF1, IBA1, allograft inflammatory factor 1 , DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule

orb1247863 (0.5 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247863 (2 ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Quick Database Links

Gene Symbol

AIF1

UniProt

P55008

RefSeq (Protein):NP_001614.3