AIF1 Antibody

• Catalog Number: orb1247830
• Category: Antibodies
• Description: AIF1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-YEEKAREKEKP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.IHC:In paraffin embedded Human Cerebellum shows staining of astrocytes. Recommended concentration, 2-4µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10µg/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: AIF1
• UniProt ID: P55008
• NCBI: NP_001614.3, NP_116573.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: AIF1, IBA1, allograft inflammatory factor 1, DASS-
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.IHC:In paraffin embedded Human Cerebellum shows staining of astrocytes. Recommended concentration, 2-4µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10µg/ml.
• Expiration Date: 12 months from date of receipt.

orb1247830 (4 μg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1247830 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.