AIF1 Antibody

SKU: orb1247830

Description

AIF1 Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.IHC:In paraffin embedded Human Cerebellum shows staining of astrocytes. Recommended concentration, 2-4µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10µg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-YEEKAREKEKP
• Target: AIF1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

AIF1, IBA1, allograft inflammatory factor 1, DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule

orb1247830 (4 μg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1247830 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

AIF1

UniProt

P55008

RefSeq (Protein):NP_116573.1, NP_001614.3