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Item 1 of 8
Item 1 of 8
AIF/Iba1 Antibody
Catalog Number: orb1294405
| Catalog Number | orb1294405 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Iba1 |
| Target | AIF / Iba1 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit-IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:300 Immunohistochemistry (Paraffin) 1:100-1:300 Immunohistochemistry (Frozen) 1:100-1:300 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only |
Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded Rat brain tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Iba1/AIF1 antibody - at 1/500 dilution, Lysates at 45 μg per lane, This blot was produced using a 15% SDS-PAGE. PVDF membrane was then blocked with 3% BSA for an hour before being incubated with orb1294405 overnight at 4°C.
Frozen sectioned for immunohistochemical analysis. Sample: Frozen sectioned Rat brain. Green: Iba1 antibody (orb1294405); Red: TUJ1 antibody; Blue: DAPI.
Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: Iba1 antibody (orb1294405): 1-200; Anti-rabbit 488: 1-500. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical of frozen sections. Sample: mouse hippocampus. Green: Iba1 antibody (orb1294405): 1-200; Anti-rabbit 488: 1-500. DAPI (blue) was used as the nuclear counter stain.
