AIF / Iba1

• Catalog Number: orb1294405
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Iba1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:300 Immunohistochemistry (Paraffin) 1:100-1:300 Immunohistochemistry (Frozen) 1:100-1:300
• Conjugation: Unconjugated
• Target: AIF / Iba1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Rat brain tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Iba1/AIF1 antibody - at 1/500 dilution, Lysates at 45 μg per lane, This blot was produced using a 15% SDS-PAGE. PVDF membrane was then blocked with 3% BSA for an hour before being incubated with orb1294405 overnight at 4°C.

Frozen sectioned for immunohistochemical analysis. Sample: Frozen sectioned Rat brain. Green: Iba1 antibody (orb1294405); Red: TUJ1 antibody; Blue: DAPI.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: Iba1 antibody (orb1294405): 1-200; Anti-rabbit 488: 1-500. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical of frozen sections. Sample: mouse hippocampus. Green: Iba1 antibody (orb1294405): 1-200; Anti-rabbit 488: 1-500. DAPI (blue) was used as the nuclear counter stain.