AIFM1 Antibody (N-term)

• Catalog Number: orb1928211
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22966
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:200, IF: 1:200, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 66901 Da
• Target: This AIFM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human AIFM1.
• UniProt ID: O95831
• NCBI: NP_004199.1, NP_001124319.1, NP_001124318.1, NP_665812.1, NP_665811.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Apoptosis-inducing factor 1, mitochondrial, 111-,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AIFM1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). AIFM1 (arrow) was detected using the purified Pab.

AIFM1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with AIFM1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent image of U251 cells stained with AIFM1 (N-term) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AIFM1 (N-term) primary antibody (1: 200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min).

Fluorescent confocal image of U251 cells stained with AIFM1 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AIFM1 (N-term) primary antibody (1: 200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). AIFM1 (N-term) immunoreactivity is localized to the cytoplasm of U251 cells.