Adiponectin Antibody

• Catalog Number: orb1239163
• Category: Antibodies
• Description: Adiponectin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Adiponectin antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human adiponectin.The immunogen is located within the last 50 amino acids of Adiponectin.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 27 kDa Observed: 26 kDa
• Target: ADIPOQ
• UniProt ID: Q15848
• NCBI: NP_004788
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Adiponectin Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Adiponectin Antibody: ACDC, ADPN, APM1, APM-1, GBP
• Note: For research use only
• Application notes: Adiponectin antibody can be used for the detection of adiponectin by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of adiponectin in HL60 cell lysate with adiponectin antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Immunohistochemistry of adiponectin in rat brain tissue with adiponectin antibody at 10 µg/mL.

Immunofluorescence of Adiponectin in Rat Brain cells with Adiponectin antibody at 10 µg/mL.

Immunohistochemistry of Adiponectin in mouse brain tissue with Adiponectin antibody at 5 µg/ml.

Immunofluorescence of Adiponectin in mouse brain tissue with Adiponectin antibody at 20 µg/mL. Red: Adiponectin Antibody (orb1239163) Blue: DAPI staining.