ADIPOR2 Antibody (Center)

• Catalog Number: orb1930664
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ADIPOR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-72 amino acids from the Central region of human ADIPOR2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q86V24
• MW: 43884 Da
• Tested applications: FC, IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:2000, WB: 1:1000, WB: 1:1000, FC: 1:25
• Antibody Type: Primary Antibody
• Clone Number: RB23213
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Adiponectin receptor protein 2, Progestin and adip
• Note: For research use only
• NCBI: NP_078827.2

Confocal immunofluorescent analysis of ADIPOR2 Antibody (Center) with Hela cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Overlay histogram showing U-2 OS cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western blot analysis of ADIPOR2 Antibody (Center) in Hela cell line lysates (35 ug/lane).ADIPOR2 (arrow) was detected using the purified Pab.

Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human liver tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human skeletal muscle tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ADIPOR2 Antibody (Center) at 1:2000 dilution. Lane 1: U-251 MG whole cell lysate. Lane 2: U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.