Adiponectin Rabbit Polyclonal Antibody

• Catalog Number: orb10048
• Category: Antibodies
• Description: Adiponectin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Adiponectin (151-244/244aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 25 kDa
• Target: ADIPOQ
• UniProt ID: Q15848
• RRID: AB_10750873
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 30 kDa adipocyte complement related protein; 30 kD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Adiponectin (orb10048) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 25 kD, Observed band size: 27 kD.

Sample: Liver (Rat) Lysate at 40 ug, Intestine (Mouse) Lysate at 40 ug, Primary: Anti-Adiponectin (orb10048) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 25 kD, Observed band size: 25 kD.

Sample: muscle (Mouse) Lysate at 40 ug, Primary: Anti-Adiponectin (orb10048) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.

Tissue/cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Adiponectin Polyclonal Antibody, Unconjugated (orb10048) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/cell: mouse liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Adiponectin Polyclonal Antibody, Unconjugated (orb10048) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.