Adiponectin receptor 2 Rabbit Polyclonal Antibody

• Catalog Number: orb10047
• Category: Antibodies
• Description: Adiponectin receptor 2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Gallus, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Adiponectin receptor 2 (315-340/386aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: ADIPOR2
• UniProt ID: Q86V24
• RRID: AB_10747019
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Adipo R2; Progestin and adipoQ receptor family mem
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: HepG2 (blue). Primary Antibody: Rabbit Anti-Adiponectin receptor 2 antibody (orb10047, Green), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (orb10047, 0.2 µg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (orb10047) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Heart (Mouse) Lysate at 40 ug, Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug, Lane 3: Heart (Rat) Lysate at 40 ug, Lane 4: Skeletal muscle (Rat) Lysate at 40 ug, Lane 5: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-Adiponectin receptor 2 (orb10047) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 43 kD.

Tissue/cell: rat pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (orb10047) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.