ZIC5 Antibody

• Catalog Number: orb1246766
• Category: Antibodies
• Description: ZIC5 Antibody
• Target: ZIC5
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-EIYGNPEVVRTIH
• UniProt ID: Q96T25
• Tested applications: ELISA, IHC
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 45kDa band in human brain lysates at 1ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 73kDda according to NP_149123.Immunohistochemistry:Paraffin embedded Human Small Intestine, Skin and Prostate. Recommended concentration: 3.75ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ZIC5, zinc finger protein of the cerebellum 5, zin
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_149123.2

orb1246766 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246766 (3.75 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246766 (3.75 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.