ZIC1/2/3/4/5 Antibody

• Catalog Number: orb304667
• Category: Antibodies
• Description: The ZIC1/2/3/4/5 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZIC1/2/3/4/5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Gallus, Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZIC1/2/3/4/5. The exact sequence is proprietary.
• UniProt ID: Q15915, Q8N9L1, Q62521, Q61467, Q96T25, O60481, O95409, Q62520, P46684
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ZIC1; ZIC; ZNF201; Zinc finger protein ZIC 1; Zinc
• Research Area: Developmental Biology
• Note: For research use only
• Entrez: 22771, 7547, 22773, 84107, 7545, 85416, 22774, 7546

Western blot analysis of ZIC1/2/3/4/5 expression in rat brain (A) whole cell lysates. (Predicted band size: 48; 55; 50; 36; 68 kD; Observed band size: 55 kD)

Immunofluorescent analysis of ZIC1/2/3/4/5 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.