ZBP1 Antibody

• Catalog Number: orb1238568
• Category: Antibodies
• Description: ZBP1 Antibody
• Target: ZBP1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: ZBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: ZBP1 Antibody is affinity chromatography purified via peptide column.
• Immunogen: Anti-ZBP1 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human ZBP1. The immunogen is located within the last 50 amino acids of ZBP1.
• UniProt ID: Q9H171
• MW: Predicted: 46 kD Observed: 46 kD
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Application notes: WB: 1-2 μg/mL; IHC-P/IF: 5-20 μg/mL; IHC-P: 1-5 μg/mL; ICC/IF: 10-20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human and rat samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures
• Alternative names: ZBP1 Antibody: DAI, DLM1, DLM-1, C20orf183, Z-DNA-
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: EAW75510

WB Validation in Human Tonsil. Loading: 10 μg of lysate. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Brain. Loading: 10 μg of lysates per lane. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Brain. Loading: 10 μg of lysates per lane. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Brain. Loading: 10 μg of lysate. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in A549 Cells. Loading: 10 μg of lysate. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of ZBP1 in Human Tonsil. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZBP1 antibody (orb1238568) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of ZBP-1 in Mouse Brain. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ZBP1 antibody (orb1238568) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of ZBP1 in Human K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 labeling ZBP1 with orb1238568 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of ZBP1 in Rat Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling ZBP1 with orb1238568 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).