ZBP1 Antibody

SKU: orb1238568

Description

ZBP1 Antibody

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  ZBP1 antibody can be used for the detection of ZBP1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ZBP1 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human ZBP1.The immunogen is located within the last 50 amino acids of ZBP1.
• Target: ZBP1
• Molecular Weight: Predicted: 39, 45 kDa Observed: 42 kDa
• Purification: ZBP1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures
• Form/Appearance: Liquid
• Buffer/Preservatives: ZBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ZBP1 Antibody: DAI, DLM1, DLM-1, C20orf183, Z-DNA-binding protein 1, Tumor stroma and activated macrophage protein DLM-1

WB Validation in Human Tonsil. Loading: 10 μg of lysate. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Brain. Loading: 10 μg of lysates per lane. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Brain. Loading: 10 μg of lysates per lane. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Brain. Loading: 10 μg of lysate. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in A549 Cells. Loading: 10 μg of lysate. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of ZBP1 in Human Tonsil. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZBP1 antibody (orb1238568) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of ZBP-1 in Mouse Brain. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ZBP1 antibody (orb1238568) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of ZBP1 in Human K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 labeling ZBP1 with orb1238568 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of ZBP1 in Rat Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling ZBP1 with orb1238568 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Quick Database Links

Gene Symbol

ZBP1

UniProt

Q9H171

RefSeq:EAW75510