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RHOU Antibody

SKU: orb1246777

Description

RHOU Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA
Predicted ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 22kDa band in lysates of cell lines A431, HeLa and HEK293 after 0.3µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 28.2kDa according to NP_067028.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: PPQQGDPAFPDRCEA
TargetRHOU
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

RHOU, ras homolog gene family, member U, ARHU, WRCH1, hG28K, WRCH-1, CDC42L1, FLJ10616, DJ646B12.2, fJ646B12.2, 2310026M05Rik, ras homolog gene family, member U, Ryu GTPase, CDC42-like GTPase, GTP-binding protein like 1, Wnt-1 responsive Cdc42 homolog, FLJ10616, WRCH1, GTP-binding protein SB128, GTP-binding protein like 1, ras-like gene family member U

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

RHOU Antibody (orb1246777)

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Available Sizes

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0.1 mg
$ 750.00
DispatchUsually dispatched within 5-10 working days
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