WRCH1 Polyclonal Antibody

SKU: orb1418108

Description

Rabbit polyclonal antibody to WRCH1.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Reactivity: Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Target: RHOU (HGNC:17794)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rho-related GTP-binding protein RhoU, CDC42-like GTPase 1, GTP-binding protein-like 1, Rho GTPase-like protein ARHU, Ryu GTPase, Wnt-1 responsive Cdc42 homolog 1, WRCH-1, RHOU (HGNC:17794)

Sample: Cerebrum (Mouse) Lysate at 40 ug. Primary: Anti- WRCH1 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 29 kD. Observed band size: 29 kD.

Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WRCH1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WRCH1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

RHOU (HGNC:17794)

UniProt

Q7L0Q8