WNT4 Antibody

SKU: orb1247870

Description

WNT4 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40+35kDa band observed in lysates of cell line Jurkat (calculated MW of 39.1kDa according to NP_110388.2). Recommended concentration: 1-3µg/mL. Primary incubation 1 hour at room temperature. Negative Control: U937 cell lysate.Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa and MCF7 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SNWLYLAKLSSVGS
• Target: WNT4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

WNT4, wingless-type MMTV integration site family, member 4, WNT-4, WNT-4 protein precursor, RP1-224A6.7, SERKAL, WNT-4 protein

orb1247870 (1 ug/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247870 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).

orb1247870 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).

Quick Database Links

Gene Symbol

WNT4

UniProt

P56705

RefSeq (Protein):NP_110388.2