WNT4 Antibody

• Catalog Number: orb1247870
• Category: Antibodies
• Description: WNT4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SNWLYLAKLSSVGS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40+35kDa band observed in lysates of cell line Jurkat (calculated MW of 39.1kDa according to NP_110388.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: U937 cell lysate.Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa and MCF7 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: WNT4
• UniProt ID: P56705
• NCBI: NP_110388.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: WNT4, wingless-type MMTV integration site family,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40+35kDa band observed in lysates of cell line Jurkat (calculated MW of 39.1kDa according to NP_110388.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: U937 cell lysate.Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa and MCF7 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247870 (1 ug/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247870 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).

orb1247870 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).