WNT4 Antibody (C-term)

• Catalog Number: orb1929887
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19966
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 39052 Da
• Target: This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-269 amino acids from the C-terminal region of human WNT4.
• UniProt ID: P56705
• NCBI: NP_110388.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Protein Wnt-4, WNT4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

(LEFT) Formalin-fixed and paraffin-embedded human lung carcinoma reacted with WNT4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT) Flow cytometric analysis of 293 cells using WNT4 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of WNT4 antibody (C-term) in 293 cell line lysates (35 ug/lane). WNT4 (arrow) was detected using the purified Pab.