VLDLR Antibody / VLDL Receptor

• Catalog Number: orb750055
• Category: Antibodies
• Description: Very Low Density Lipoprotein Receptor (VLDLR/VLDL receptor) is a member of the LDL receptor gene family which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR associates with RAP (receptor associated protein) during receptor folding, and RAP facilitates the secretion of the extracellular region of VLDLR. VLDL receptor is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein), which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VLDLR/1337
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, lambda
• Immunogen: A human recombinant partial protein from the C-terminal region was used as the immunogen for this VLDL Receptor antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This VLDL Receptor antibody is available for research use only.
• Entrez: 7436
• UniProt ID: P98155
• Storage: Store the VLDL Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the VLDL Receptor antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pancreas with VLDL Receptor antibody (clone VLDLR/1337). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.