VLDL Receptor Rabbit Polyclonal Antibody

SKU: orb4608

Description

VLDL Receptor Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ICC
• Dilution Range: ICC/IF=1:50-200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human VLDL Receptor (551-650/873aa)
• Target: VLDLR
• Molecular Weight: 93 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Very low density lipoprotein receptor; VLDL R; VLDLR; VLDL-R; VLDLRCH.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (VLDL Receptor) polyclonal Antibody, Unconjugated (orb4608) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: 293T (Human) Cell Lysate at 30 ug, Lane 3: A431 (Human) Cell Lysate at 30 ug, Lane 4: SiHa (Human) Cell Lysate at 30 ug, Primary: Anti-VLDL Receptor (orb4608) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

Quick Database Links

Gene Symbol

VLDLR

UniProt

P98155