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VLDLR Antibody (Center)

Catalog Number: orb1930371

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1930371
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB21297
Tested applicationsFC, IHC-P, WB
Predicted ReactivityRabbit
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeWB: 1:2000, WB: 1:2000, IHC-P: 1:10~50, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW96098 Da
TargetThis VLDLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-510 amino acids from the Central region of human VLDLR.
UniProt IDP98155
NCBINP_001018066.1, NP_003374.3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesVery low-density lipoprotein receptor, VLDL recept
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NoteFor research use only
Expiration Date12 months from date of receipt.
VLDLR Antibody (Center)

Staining VLDLR in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

VLDLR Antibody (Center)

Staining VLDLR in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

VLDLR Antibody (Center)

Overlay histogram showing THP-1 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

VLDLR Antibody (Center)

VLDLR Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VLDLR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

VLDLR Antibody (Center)

Anti-VLDLR Antibody (Center) at 1:2000 dilution + human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 96 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

VLDLR Antibody (Center)

Anti-VLDLR Antibody (Center) at 1:2000 dilution + 293T/17 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 96 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

  • VLDLR Antibody (Center) [orb1166560]

    FC,  IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 30 μl