Recombinant Vimentin Antibody

SKU: orb2636746

Description

Anti-Vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Bovine, Canine, Equine, Feline, Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the recombinant Vimentin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Clone No.: VIM/6430R
• Immunogen: A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant Vimentin antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P08670