Vimentin Mouse mAb

• Catalog Number: orb763489
• Category: Antibodies
• Description: Mouse monoclonal antibody to Vimentin
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1mg/mL
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
• Immunogen: Recombinant Protein
• UniProt ID: P08670
• MW: 50-57kDa
• Tested applications: IF, IHC, WB
• Dilution range: WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-FLJ36605 antibody antibody, anti-VIM antibody
• Note: For research use only
• Entrez: 7431
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain tissue with orb763489 diluted at 1:2, 000.

IF analysis of Hela with orb763489 (Left) and DAPI (Right) diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Vimentin (orb763489) Mouse mAb diluted at 1:500.

Western blot analysis of extracts from MCF-7 using Vimentin diluted at 1:3, 000.