Recombinant rabbit vimentin Antibody

SKU: orb669777

Description

Rabbit monoclonal antibody to Vimentin

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ICC, IHC, WB
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: RV205
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Indirect immunofluorescence staining of normal human dermal fibroblasts in tissue culture with orb669777 (diluted 1:100), showing the specific cytoskeletal pattern of vimentin intermediate filaments.

Indirect immunofluorescence staining of human kidney tissue section with orb669777 (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Indirect immunofluorescence staining of human kidney tissue section with orb669777 (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue section of placenta with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue section of testis with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, endothelial cells in blood vessels and Sertoli cells.

Immunostaining of human paraffin embedded tissue sections of human colon with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human kidney with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and podocytes. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human small intestine with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human spleen with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types.

Non-reduced and reduced SDS-PAGE of orb669777 showing its purity.

Quick Database Links

UniProt

P08670