Recombinant rabbit vimentin Antibody

• Catalog Number: orb669777
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Vimentin
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• UniProt ID: P08670
• Tested applications: ICC, IHC, WB
• Antibody Type: Primary Antibody
• Clone Number: RV205
• Source: This product is a recombinant rabbit anti vimentin monoclonal antibody produced in mammalian HEK293 cells. Clone RV205 is a reformatted antibody (species switch), produced by cloning the antibody sequence of the mouse anti vimentin hybridoma RV202, which was derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer Biology, Cardiovascular Research, Cell Biol
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Indirect immunofluorescence staining of normal human dermal fibroblasts in tissue culture with orb669777 (diluted 1:100), showing the specific cytoskeletal pattern of vimentin intermediate filaments.

Indirect immunofluorescence staining of human kidney tissue section with orb669777 (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Indirect immunofluorescence staining of human kidney tissue section with orb669777 (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue section of placenta with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue section of testis with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, endothelial cells in blood vessels and Sertoli cells.

Immunostaining of human paraffin embedded tissue sections of human colon with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human kidney with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and podocytes. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human small intestine with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue. As expected, no reactivity is seen in the epithelial cell compartment.

Immunostaining of human paraffin embedded tissue sections of human spleen with orb669777 (diluted 1:200), showing the specific pattern of vimentin in the mesenchymal cell types.

Non-reduced and reduced SDS-PAGE of orb669777 showing its purity.