Vimentin Antibody

SKU: orb749844

Description

This mAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the anti-Vimentin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: VM1170
• Immunogen: Recombinant full-length human protein was used as the immunogen for the anti-Vimentin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human melanoma stained with anti-Vimentin antibody (clone VM1170).

IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with anti-Vimentin antibody (clone VM1170).

IHC: Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with anti-Vimentin antibody (clone VM1170).

IHC: Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with anti-Vimentin antibody (clone VM1170).

IHC: Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with anti-Vimentin antibody (clone VM1170).

IHC: Formalin-fixed, paraffin-embedded human uterus stained with anti-Vimentin antibody (clone VM1170).

Western blot testing of human HepG2 cell lysate with anti-Vimentin antibody. Predicted molecular weight ~58 kDa.

Quick Database Links

UniProt

P08670