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Featured
KO/KD
Validated
Validated
Description
Research Area
Musculoskeletal & Connective Tissue Research
Images & Validation
−
Item 1 of 13
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB=1:1000-10000, IHC-P=1:200-1000, IHC-F=1:200-1000, ICC/IF=1:100-500, IF=1:200-1000, Flow-Cyt=1ug/Test |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | B5D9 |
| Immunogen | Recombinant human Vimentin Protein |
| Target | VIM |
| Molecular Weight | 53 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−VIME_HUMAN; VIM; VIME_MOUSE; VIME_RAT; vimentin
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Quick Database Links
Gene Symbol
VIM
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Filter by Applications
Filter by Species
Elham Hashemi 1, Mansoureh Movahedin 2, Ali Ghiaseddin 3, Seyed Mohammad Kazem Aghamir In Vitro Spermatogenesis on Human Decellularized Testicular Matrix Plates Following Exosome Treatment in a Dynamic Culture System Stem Cell Rev Rep, (2024)
Applications
IF
Reactivity
Human
Ahmed M Abou-Shanab # 1 2, Shaimaa Shouman # 1 2, Alaa E Hussein 1 2, Ola A Gaser 1 2, Shireen Magdy 1 2, Eman Ashraf 1 2, Radwa Ayman Salah 1 2, Omaima Idris 3, Nagwa El-Badri 4 Amniotic membrane promotes doxorubicin potency by suppressing SH-SY5Y neuroblastoma cell angiogenesis BMC Cancer, (2025)
Applications
WB
Reactivity
Human
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Free Secondary Antibody (20 ul)0/0
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