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Vimentin Antibody

Catalog Number: orb385770

DispatchUsually dispatched within 5-10 working days
$ 764.00
Catalog Numberorb385770
CategoryAntibodies
DescriptionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
Species/HostMouse
ClonalityMonoclonal
Clone NumberVIM66-1
Tested applicationsIHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenFull length human recombinant protein was used as the immunogen for the Vimentin antibody.
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Vimentin antibody is available for research use only.
UniProt IDP08670
StorageStore the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesTitering of the Vimentin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
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    Polyclonal

    Unconjugated

    50 μg, 100 μg
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