Vimentin Antibody

SKU: orb1332453

Description

Mouse monoclonal antibody to Vimentin (VIM)

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB 1:2000
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: OTI6B12
• Immunogen: Human recombinant protein fragment corresponding to amino acids 210-466 of human VIM (NP_003371) produced in E.coli.
• Target: Vimentin
• Molecular Weight: 53.5 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (Right lane) cDNA for 48 hrs and lysed using Vimentin (VIM) antibody

Quick Database Links

Gene Symbol

Vimentin

UniProt

P08670

RefSeq (mRNA):NM_003380