Vimentin Antibody

SKU: orb1248094

Description

Vimentin Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates (calculated MW of 53.7kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1). Recommended concentration: 0.1-2µg/mL.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10µg/mL. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended concentration: 5µg/mL. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QVINETSQHHDDLE
• Target: Vimentin
• Molecular Weight: Approx 55kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VIM, vimentin, FLJ36605

Quick Database Links

Gene Symbol

Vimentin

UniProt

P08670

RefSeq (Protein):NP_003371.2