V5 Tag Antibody

SKU: orb1927580

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:1000

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

The V5 tag derived from the P and V protein of the simian virus 5 (SV5, a paramyxovirus).

Western blot analysis of anti-V5 Tag Antibody in 12tag protein (35 ug/lane). 12 Tag (arrow) was detected using the purified Pab.

Lanes: 1: non-transfected cells;.2: V5-tagged empty plasmid;.3: protein A (V5-tagged);.4: protein B (V5-tagged);.5: protein C (V5-tagged);.6: protein D (V5-tagged);

All lanes: Anti-Tag-V5 at 1:2000 dilution + recombinant Protein lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 50KDa. Blocking/Dilution buffer: 5% NFDM/TBST.