V5 Tag Antibody

• Catalog Number: orb1927580
• Category: Antibodies
• Description: V5 Tag Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Tested applications: WB
• Dilution range: WB - 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-V5 Tag Antibody in 12tag protein (35 ug/lane). 12 Tag (arrow) was detected using the purified Pab.

Lanes: 1: non-transfected cells;.2: V5-tagged empty plasmid;.3: protein A (V5-tagged);.4: protein B (V5-tagged);.5: protein C (V5-tagged);.6: protein D (V5-tagged);

All lanes: Anti-Tag-V5 at 1:2000 dilution + recombinant Protein lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 50KDa. Blocking/Dilution buffer: 5% NFDM/TBST.