V5 tag Rabbit Polyclonal Antibody

SKU: orb13472

Description

V5 tag Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB=1:10000-100000, ELISA=1:1000-5000
• Reactivity: All

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated V5 tag
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

V5-tag; GKPIPNPLLGLDST epitope tag; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope tag.

Sample: Lane 1: V5-Tagged Fusion Protein Overexpression E.coli Lysate at 10ug, Primary: Anti-V5 tag (orb13472) at 1/100000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kDa, Observed band size: 45 kDa.

Sample: Lane 1: V5-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug Lane 2: V5-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug Primary: Anti-V5 tag (orb13472) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD.