V5-Tag Mouse mAb

• Catalog Number: orb763375
• Category: Antibodies
• Description: Mouse monoclonal antibody to V5-Tag
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Concentration: 1mg/mL
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
• Immunogen: Synthetic Peptide
• Tested applications: IF, IP, WB
• Dilution range: WB 1:5,000 IP 1:200 IF 1:1,000
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IP antibody use: 5ug V5 Mouse IgG1 per ml Lysate, WB 1:5000 1: untransfected 293 cell lysate 2: transfected 293 cell lysate with V5-tag fusion protein 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose) 4: IP (transfected 293 + normal Mouse IgG + Protein G ag).

IF analysis of 293 cells transfected with a V5-tag protein, using ELKbio anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5).

1ug V5 fusion protein + Primary antibody dilution at 1: 1:5, 000 2: 1:10, 000.