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Description
Images & Validation
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Item 1 of 1
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB:1:500-1:5,000, IF:1:50-1:2,000 |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Antigen: Purified recombinant peptide produced in E. coli. Antigen Sequence: MGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPGRLERPHRD |
| Target | V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation. |
| Purification | Epitope affinity purified |
| Conjugation | Unconjugated |
Customer Validated Data
− The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution
Latest Experiments
1 ResultsDilution
Sample
Summary
IP
Human
-
HEK293A cell lysates expressing V5‑connexin constructs
Cells are lysed in buffer containing...
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Polyclonal antibody supplied as 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GKPIPNPLLGLDST epitope, V5 tag antibody.
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Quick Database Links
Gene Symbol
V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.
Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
Available Sizes
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