Ubiquitin Antibody

SKU: orb699399

Description

Mouse monoclonal antibody to Ubiquitin

Research Area

Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution Range: WB (1:10000), ICC/IF (1:100)
• Reactivity: All
• Application Notes:
  A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone No.: FK1
• Immunogen: Ubiquitin conjugated lysozyme
• Target: Ubiquitin
• Purification: Gel filtration
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.

Western Blot analysis of Poly-Ubiquitin showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Lane 1: MW ladder. Lane 2: Poly-Ubiquitin, K48-linked (2 ug). Lane 2: Poly-Ubiquitin, K63-linked (0.6 ug). Load: 5 uL. Block: 1% BSA in TBST. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:2000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Quick Database Links

Gene Symbol

Ubiquitin

UniProt

P0CG47

NCBI Gene ID

7314

RefSeq:BAC56955.1