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Ubiquitin Antibody

SKU: orb413657

Description

Rabbit Recombinant monoclonal antibody to Ubiquitin

Research Area

Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeWB (1:1000); ELISA (1:1000)
ReactivityAll
Application Notes
A 1:1000 dilution was sufficient for detection of Ubiquitin in 2 µg of purified poly-ubiquitin chains (Poly-Ubiquitin: Ub1-Ub7 WT Chains K63-linked, k48-linked) by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

Antibody TypeRecombinant Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG
Clone No.FK2
ImmunogenUbiquitin conjugated lysozyme
TargetUbiquitin
PurificationProtein A Purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 650.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-3 weeks
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