Ubiquitin Antibody

• Catalog Number: orb413657
• Category: Antibodies
• Description: Rabbit Recombinant monoclonal antibody to Ubiquitin
• Target: Ubiquitin
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: All
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Protein A Purified
• Immunogen: Ubiquitin conjugated lysozyme
• UniProt ID: P0CG47, P0CG48
• Tested applications: ELISA, WB
• Dilution range: WB (1:1000); ELISA (1:1000)
• Application notes: A 1:1000 dilution of SMC-550 was sufficient for detection of Ubiquitin in 2 µg of purified poly-ubiquitin chains (Poly-Ubiquitin: Ub1-Ub7 WT Chains K63-linked, k48-linked) by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Antibody Type: Recombinant Antibody
• Clone Number: FK2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Polyubiquitin B antibody, RPS27A antibody, UBA52 a
• Research Area: Neuroscience, Protein Biochemistry, Signal Transdu
• Note: For research use only
• Entrez: 7314

Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Rabbit Anti-Ubiquitin Monoclonal Antibody, Clone FK2. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG:HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.