Ubiquitin Antibody

SKU: orb413621

Description

Rabbit Recombinant monoclonal antibody to Ubiquitin

Research Area

Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB (1:1000); ELISA (1:1000)
• Reactivity: All
• Application Notes:
  A 1:1000 dilution of SMC-548 was sufficient for detection of Ubiquitin in 2 µg of purified poly-ubiquitin chains (Poly-Ubiquitin: Ub1-Ub7 WT Chains K63-linked, k48-linked) by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: RGL3R
• Immunogen: Ubiquitin conjugated lysozyme
• Target: Ubiquitin
• Purification: Protein A Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B

Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Rabbit Anti-Ubiquitin Monoclonal Antibody, Clone RGL3R. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG:HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.

Quick Database Links

Gene Symbol

Ubiquitin

UniProt

P0CG47, P0CG48

NCBI Gene ID

7314