Ubiquitin Antibody

• Catalog Number: orb396226
• Category: Antibodies
• Description: Mouse Anti-Human Ubiquitin Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FK2
• Tested applications: ELISA, ICC, IF, IHC, IP, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: Ubiquitin conjugated lysozyme
• Concentration: 1 mg/ml
• Dilution range: WB (1:10000)
• Conjugation: Unconjugated
• Target: Ubiquitin
• Entrez: 7314
• UniProt ID: P0CG47
• NCBI: BAC56955.1
• Storage: -20°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: Polyubiquitin B Antibody, RPS27A Antibody, UBA52 A
• Note: For research use only
• Application notes: A 1:5000 dilution of SMC-214 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.