TUBB3 Antibody

• Catalog Number: orb1248640
• Category: Antibodies
• Description: TUBB3 Antibody
• Target: TUBB3
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-DPSGNYVGDSD
• UniProt ID: Q13509
• MW: Approx 52 kDa
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 52kDa band observed in lysates of cell line HepG2 (calculated MW of 50.4kDa according to NP_006077.2). Recommended concentration: 1-3ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TUBB3, tubulin, beta 3, CDCBM, CFEOM3A, TUBB4, bet
• Note: For research use only
• NCBI: NP_006077.2

orb1248640 (2 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.