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Catalog Number | orb44544 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to Tubulin beta 3 |
Clonality | Monoclonal |
Clone Number | TU-20 |
Tested applications | FC, ICC, IHC-P, WB |
Reactivity | Canine, Human, Mouse, Porcine, Rat |
Isotype | Mouse IgG1 |
Immunogen | Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | betaIII-Tubulin |
Entrez | 10381 |
UniProt ID | Q13509 |
RRID | AB_10990257 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | anti-beta 3 tubulin antibody, anti-beta-4 antibody Read more... |
Note | For research use only |
Application notes | Western blotting: Recommended dilution: 1-2 μg/ml; positive control: porcine brain lysate; negative control: HPB-ALL human peripheral blood leukemia cell line; reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; positive tissue: neuronal tissue. Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Sana, Jiri et al. Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival Sci Rep, 8, 2836 (2018)
Separation of MCF-7 cells stained using anti-betaIII-Tubulin (TU-20) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
Immunocytochemistry staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 µg/ml). Nuclei were stained with DAPI (blue).
Immunocytochemistry staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). B - Merged image of co-staining with anti-beta-tubulin (TU-06; green; cat. no. orb44535). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).
Immunohistochemistry staining of human brain (paraffin sections) using anti-betaIII tubulin (TU-20).
Immunohistochemistry staining of betaIII tubulin (red) in tissue sections of murine brain expressing GFP in some of its neurons (green). Mouse monoclonal antibody TU-20 (anti-betaIII tubulin) was detected with goat anti-mouse IgG1 conjugated with Alexa Fluor 555.
Western blotting analysis of human betaIII-tubulin using mouse monoclonal antibody TU-20 on lysates of U87 cells and Neuro 2a cells (and Jurkat cells as a negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-betaIII-tubulin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for betaIII-tubulin at approximately 55 kDa.