betaIII-Tubulin Antibody

SKU: orb44544

Description

Mouse monoclonal antibody to Tubulin beta 3

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, ICC, IHC-P, WB
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Application Notes:
  Western blotting: Recommended dilution: 1-2 μg/ml; positive control: porcine brain lysate; negative control: HPB-ALL human peripheral blood leukemia cell line; reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; positive tissue: neuronal tissue. Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: TU-20
• Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
• Target: betaIII-Tubulin
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TUBB3

Quick Database Links

Gene Symbol

betaIII-Tubulin

UniProt

Q13509

NCBI Gene ID

10381

Sana, Jiri et al. Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival Sci Rep, 8, 2836 (2018)

PubMed: 29434344