TUBB3 (Neuronal Marker) Mouse Monoclonal Antibody

• Catalog Number: orb500843
• Category: Antibodies
• Description: TUBB3 (Neuronal Marker) Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6F12
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TUBB3
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: TUBB3
• UniProt ID: Q13509
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tubulin beta 3; beta III Tubulin; Tubb3; Tubulin b
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (orb500843) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (orb500843) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (orb500843) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TUBB3) Polyclonal Antibody, Unconjugated (orb500843) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (orb500843) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (TUBB3 (Neuronal Marker)) Monoclonal Antibody, Unconjugated (orb500843) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TUBB3 (Neuronal Marker) Monoclonal Antibody, Unconjugated (orb500843) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-TUBB3 (orb500843) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Lane 1: Human A549 cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human U251 cell lysates, Lane 4: Human U87MG cell lysates, Primary: Anti-TUBB3 (orb500843) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 50 kDa, Observed band size: 47 kDa.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-TUBB3 (orb500843) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.