TUBB3 Antibody [5D5D2]

• Catalog Number: orb1258462
• Category: Antibodies
• Description: TUBB3 Antibody [5D5D2]
• Target: TUBB3
• Clonality: Monoclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant protein of human TUBB3.
• UniProt ID: Q13509
• MW: Observed: 55kDa
• Tested applications: WB
• Application notes: WB: 1:500 - 1:2000
• Antibody Type: Primary Antibody
• Clone Number: [5D5D2]
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: beta-4, CDCBM, CFEOM3A, class III beta-tubulin, OT
• Research Area: Cell Biology, Neuroscience, Signal Transduction
• Note: For research use only

Western blot analysis of extracts of various cell lines, using TUBB3 antibody (orb1258462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.