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Catalog Number | orb229789 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to TUBB1 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2A1A9 |
Tested applications | FC, ICC, IHC, WB |
Reactivity | Human, Monkey, Mouse, Rat |
Isotype | Mouse IgG1 |
Immunogen | Purified recombinant fragment of human TUBB1 (AA: 33-166) expressed in E. Coli. |
Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
Dilution range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
Conjugation | Unconjugated |
MW | 50.3kDa |
Target | TUBB1 |
Entrez | 81027 |
UniProt ID | Q9H4B7 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
Alternative names | Tubulin beta-1 chain Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HEK293 using TUBB1 antibody
Western blot analysis of K562 (Lane1), HepG2 (Lane2), A431 (Lane3), Jurkat (Lane4), Hela (Lane5), NIH/3T3 (Lane6), Cos7 (Lane7) and PC12 (Lane8) cell lysate using TUBB1 antibody
ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Flow cytometric analysis of A431 cells using TUBB1 antibody
Immunofluorescence analysis of Hela cells using TUBB1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded ovarian cancer tissues using TUBB1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded ovarian cancer tissues using TUBB1 antibody
Western blot analysis of human recombinant protein using TUBB1 antibody
ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Gallus, Guinea pig, Insect, Monkey, Porcine, Rabbit, Sheep, Yeast | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |