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Catalog Number | orb1255763 |
---|---|
Category | Antibodies |
Description | TRPA1 Antibody |
Target | TRPA1 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 850-950 of human TRPA1 (NP_015628.2). |
UniProt ID | O75762 |
MW | Observed: 128kDa |
Tested applications | IF, IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | TRPA1, ANKTM1, TRPA-1 |
Note | For research use only |
Western blot analysis of extracts of mouse liver, using TRPA1 antibody (orb1255763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using TRPA1 antibody (orb1255763) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TRPA1 antibody (orb1255763) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using TRPA1 antibody (orb1255763) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using TRPA1 antibody (orb1255763) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TRPA1 antibody (orb1255763) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TRPA1 antibody (orb1255763) at dilution of 1:100 (40x lens).
IF, IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Rabbit, Yeast | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |