TRPA1 Rabbit pAb Discontinued

SKU: orb374201

Description

Rabbit polyclonal antibody to TRPA1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution Range: WB: 1:500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human TRPA1 (NP_015628.2).
• Molecular Weight: 128kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TRPA1, ANKTM1, FEPS

Immunofluorescence analysis of paraffin-embedded Rat cerebellum tissue using TRPA1 Rabbit pAb (orb374201) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Immunohistochemistry analysis of paraffin-embedded Human stomach using TRPA1 Rabbit pAb (orb374201) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Mouse heart using TRPA1 Rabbit pAb (orb374201) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Rat brain using TRPA1 Rabbit pAb (orb374201) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western blot analysis of lysates from Mouse brain, using TRPA1 Rabbit pAb (orb374201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Quick Database Links

UniProt

O75762

Qilong Mao 1, Yu Zeng 2, Huining Liang 3, Siyu Hou 3, Qi Wang 4, Jiaxin Zhang 1, Zhou Lan 3, Hongjun Wang 5, Jing Wang 6, Hongmei Zhao 7 A novel angiotensin II type 2 receptor antagonist TDI05 alleviates the peripheral neuropathic pain Neurosci Lett ., (2025)

PubMed: 40885515

Applications

IHC

Reactivity

Rat