CD71 Antibody / Transferrin Receptor

SKU: orb750031

Description

It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: FACS, IF, IHC
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-3ug/ml,Immunohistochemistry: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD71 antibody should be determined by the researcher.HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: TFRC/1396
• Immunogen: Recombinant human protein was used as the immunogen for the CD71 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE Analysis of Purified, BSA-Free CD71 Antibody (clone TFRC/1396). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P02786