CD71 Antibody / Transferrin Receptor (Extracellular domain)

SKU: orb2640355

Description

It recognizes a ~85-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This mAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution Range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT Western blot: 0.5-1ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD71 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: TFRC/1817
• Immunogen: Amino acids 94-212 (extracellular domain) of the human protein were used as the immunogen for the Transferrin Receptor / CD71 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human placenta tissue with Transferrin Receptor / CD71 antibody (clone TFRC/1817). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human 1) Jurkat, 2) HeLa, 3) MCF7 and K562 cell lysate with CD71 antibody (clone TFRC/1817). Predicted molecular weight: 85-95 kDa.

IHC testing of FFPE human bone marrow with Transferrin Receptor / CD71 antibody (clone TFRC/1817). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free CD71 Antibody (clone TFRC/1817). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P02786