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Catalog Number | orb386209 |
---|---|
Category | Antibodies |
Description | It recognizes a ~85-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This mAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | TFRC/1817 |
Tested applications | ELISA, IHC-P |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | Amino acids 94-212 (extracellular domain) of the human protein were used as the immunogen for the Transferrin Receptor / CD71 antibody. |
Dilution range | ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT Western blot: 0.5-1ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This CD71 antibody is available for research use only. |
UniProt ID | P02786 |
Storage | Store the CD71 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the CD71 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human placenta tissue using Transferrin Receptor antibody
Western blot analysis of human Jurkat(lane 1), HeLa(lane 2), MCF7(lane 3) and K562(lane 4) cell lysate using Transferrin Receptor antibody
Immunohistochemical staining of Human bone marrow using Transferrin Receptor antibody
ELISA, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-Fr | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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