Transferrin Receptor Antibody / CD71

SKU: orb749804

Description

It recognizes a ~85-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This mAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of CD71. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Transferrin Receptor antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: TFRC/1059
• Immunogen: Recombinant human protein was used as the immunogen for the Transferrin Receptor antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry testing of human Jurkat cells with Transferrin Receptor antibody (clone TFRC/1059); Red = isotype control, Blue = Transferrin Receptor antibody.

Flow cytometry testing of Jurkat cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Transferrin Receptor antibody (TFRC/1059).

Immunofluorescent staining of PFA-fixed human K562 cells with Transferrin Receptor antibody (clone TFRC/1059, green) and Reddot nuclear stain (red).

Quick Database Links

UniProt

P02786