Transferrin Receptor Antibody / TFRC / CD71

• Catalog Number: orb749804
• Category: Antibodies
• Description: It recognizes a ~85-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This mAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of CD71. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Recombinant human protein was used as the immunogen for the Transferrin Receptor antibody.
• UniProt ID: P02786
• Tested applications: FACS, IF
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml
• Application notes: Optimal dilution of the Transferrin Receptor antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: TFRC/1059
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the Transferrin Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This Transferrin Receptor antibody is available for research use only.
• Note: For research use only

Flow cytometry testing of human Jurkat cells with Transferrin Receptor antibody (clone TFRC/1059); Red = isotype control, Blue = Transferrin Receptor antibody.

Flow cytometry testing of Jurkat cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Transferrin Receptor antibody (TFRC/1059).

Immunofluorescent staining of PFA-fixed human K562 cells with Transferrin Receptor antibody (clone TFRC/1059, green) and Reddot nuclear stain (red).