Transferrin Receptor Antibody / CD71

SKU: orb749388

Description

This antibody recognizes a ~85-95kDa protein which is identified as cell surface Transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. The antibody is highly specific to transferrin receptor and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of transferrin receptor. Its ligand is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Images & Validation

• Tested Applications: ELISA, FACS, IF
• Dilution Range: ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Transferrin receptor antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 66IG10
• Immunogen: Recombinant human protein was used as the immunogen for this Transferrin receptor antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

FACS testing of K562 cells with Alexa Fluor 488 conjugated Transferrin receptor antibody (green) and isotype control (gray).

SDS-PAGE Analysis of Purified, BSA-Free Transferrin Receptor Antibody (clone 66IG10). Confirmation of Integrity and Purity of the Antibody.