Transferrin Receptor Antibody / TFRC / CD71

• Catalog Number: orb386210
• Category: Antibodies
• Description: It recognizes a ~85-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This mAb is highly specific to Transferrin Receptor / CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin Receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Amino acids 94-212 (extracellular domain) of the human protein were used as the immunogen for the Transferrin Receptor antibody.
• UniProt ID: P02786
• Tested applications: ELISA, IHC-P
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Transferrin Receptor antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: TFRC/1818
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the Transferrin Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This CD71 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human bone marrow with CD71 / Transferrin Receptor antibody (clone TFRC/1818). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human placenta tissue with CD71 / Transferrin Receptor antibody (clone TFRC/1818). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free Transferrin Receptor Antibody (clone TFRC/1818). Confirmation of Integrity and Purity of the Antibody.