Transcription factor E2F4 Antibody

SKU: orb1248334

Description

Transcription factor E2F4 Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Porcine
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 44.0kDa according to NP_001941.2). Recommended concentration: 0.1-0.3µg/mL. Approx 40+50kDa bands observed in Pig Brain and Heart lysates (calculated MW of 43.2kDa according to Pig XP_003126981.3). Recommended concentration: 0.01-0.003µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-PKELSEIFDPTR
• Target: Transcription factor E2F4
• Molecular Weight: Approx 50kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E2F4, E2F transcription factor 4, p107/p130-binding, E2F-4, p107/p130-binding protein, transcription factor E2F4

Quick Database Links

Gene Symbol

Transcription factor E2F4

UniProt

Q16254

RefSeq (Protein):NP_001941.2