E2F4 Rabbit Polyclonal Antibody

SKU: orb10571

Description

E2F4 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human E2F4 (1-150/413aa)
• Target: E2F4
• Molecular Weight: 44 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

E2F 4; E2F transcription factor 4; E2F transcription factor 4 p107/p130 binding; p107/p130 binding protein; Transcription factor E2F 4; Transcription factor E2F4; E2F4_HUMAN.

CK18 was immunoprecipitated from human hela cells lysate with orb10571 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution. Lane 1: human hela cells lysate 10 µg (Input). Lane 2: orb10571 IP in human hela cells lysate. Lane 3: native rabbit IgG IP in human hela cells lysate (negative control). Secondary, All lanes: Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (E2F4) Polyclonal Antibody, Unconjugated (orb10571) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Human A431 cell lysates, Lane 2: Human 293T cell lysates, Lane 3: Human HL-60 cell lysates, Lane 4: Human Jurkat cell lysates, Primary: Anti-E2F4 (orb10571) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 59 kDa.

Quick Database Links

Gene Symbol

E2F4

UniProt

Q16254

References

Daniel J Merk 1 2, Foteini Tsiami 1 2, Sophie Hirsch 1 2 3, Bianca Walter 1 2, Lara A Haeusser 1 2 4, Jens D Maile 1 2, Aaron Stahl 5, Mohamed A Jarboui 6 7, Anna Lechado-Terradas 8, Franziska Klose 6 7, Sepideh Babaei 9 2, Jakob Admard 10 11 3, Nicolas Casadei 10 11 3, Cristiana Roggia 10 11 3, Michael Spohn 12 13, Jens Schittenhelm 14 3, Stephan Singer 15 2 3, Ulrich Schüller 12 16 17, Federica Piccioni 18 19, Nicole S Persky 18, Manfred Claassen 9 20 2 21, Marcos Tatagiba 22 4 3, Philipp J Kahle 8 23, David E Root 18, Markus Templin 5, Ghazaleh Tabatabai Functional screening reveals genetic dependencies and diverging cell cycle control in atypical teratoid rhabdoid tumors Genome Biol ., (2024)

PubMed: 39617889